nanoXIM HAp pastes are nano-hydroxyapatite water based pastes specially recommended to manufacture bone graft substitutes such as injectables for bone regeneration and implants for hard tissues.
The hydroxyapatite nanoparticles comprised in these products form a perfectly aligned structure of nanocrystals.
Due to the similarity between nano-hydroxyapatite and mineralized bone, nanoXIM HAp pastes have a high affinity to hard tissues, establishing chemical bonds with the host tissue.
|Promotes fast bone regeneration and an early vascularization due to their osteoconductive and osteostimulative properties|
|Encourages protein adsorption and osteoblast adhesion|
|Enhances osteoblast functions|
|Resorbable material replaced by new bone during the healing process|
|Optimal defect filling capacity due to pasty consistency|
|Hydroxyapatite phase purity (100%)|
|Hydroxyapatite nanoparticles (< 50 nm)|
|High surface area (≥ 80 m2/g)|
nanoXIM•HAp100 is a series of synthetic nano-hydroxyapatite aqueous pastes, manufactured and supplied in two different concentrations, 15 and 30 wt%.
These products comprise nano-hydroxyapatite particles with typical particle size below 50 nm in a rod-like shape (typically 30-40 nm length and 5-10 nm width) suspended in pure water.
|Reference||Hydroxyapatite (wt%)||Specific surface area,
as Pb (ppm)
|nanoXIM•HAp102||15±1.0||≥ 80||≤ 20|
|nanoXIM•HAp103||30±3.0||≥ 80||≤ 20|
High Resolution TEM of
Electron crystallography image
During bone regeneration, Human Mesenchymal Stem Cells (HMSCs) play an important role as they are recruited to the injured place and differentiate into bone cells, enabling the regeneration process.
Considering the importance of these cells, it was evaluated the biological performance of nanoXIM•HAp102 in the proliferation and osteoblastic differentiation of HMSCs.
L. Siqueira, N. Ribeiro, M.B.A. Paredes, L. Grenho, C. Cunha-Reis, E.S. Trichês, M.H. Fernandes, S.R. Sousa, F.J. Monteiro , “Influence of PLLA/PCL/HA Scaffold Fiber Orientation on Mechanical Properties and Osteoblast Behavior”, Materials, 12, 3879, doi:10.3390/ma12233879 (2019).
R.N. Salaie, A. Besinis, H. Le, C. Tredwin, R.D. Handy, “The biocompatibility of silver and hydroxyapatite coatings on titanium dental implants with human primary osteoblast cells”, Materials Science & Engineering C, doi.org/10.1016/j.msec.2019.110210 (2019).
D. Kołbuk, O. Urbanek, P. Denis, E. Choińska, “Sonochemical coating as an effective method of polymeric nonwovens functionalization”, Journal of Biomedical Materials Research – Part A, p. 65 (2019).
C. Santos, S. Turiel, P.S. Gomes, E. Costa, A. Santos Silva, P. Quadros, J. Duarte, S. Battistuzzo, M.H. Fernandes, “Vascular biosafety of commercial hydroxyapatite particles: discrepancy between blood compatibility assays and endothelial cell behavior”, Journal of Nanobiotechnology, 16(27), doi: 10.1186/s12951-018-0357-y (2018).
G. Ruphuy, T. Weide, J.C.B. Lopes, M.M. Dias, M.F. Barreiro, “Preparation of nano-hydroxyapatite/chitosan aqueous dispersions: from lab scale to continuous production using an innovative static mixer”, Carbohydrate Polymers, 202, p. 20 (2018).
G. Ruphuy, M. Souto-Lopes, D. Paiva, P. Costa, A.E. Rodrigues, F.J. Monteiro, C.L. Salgado, M.H. Fernandes, J.C. Lopes, M.M. Dias, M.F. Barreiro, “Supercritical CO2 assisted process for the production of high-purity and sterile nano-hydroxyapatite/chitosan hybrid scaffolds”, J Biomed Mater Res Part B, DOI: 10.1002/jbm.b.33903 (2017).
Y. Ryabenkova, A. Pinnock, P.A. Quadros, R.L. Goodchild, G. Möbus, A. Crawford, P.V. Hatton, C.A. Miller, “The relationship between particle morphology and rheological properties in injectable nano-hydroxyapatite bone graft substitutes”, Materials Science and Engineering: C, 75, p. 1083, (2017).
V. Hruschka, S. Tangl, Y. Ryabenkova, P. Heimel, D. Barnewitz, G. Möbus, C. Keibl, J. Ferguson, P. Quadros, C. Miller, R. Goodchild, W. Austin, H. Redl, T. Nau, “Comparison of nanoparticular hydroxyapatite pastes of different particle content and size in a novel scapula defect model”, Nature Scientific Reports 7, Article number: 43425; doi: 10.1038/srep43425 (2017).
A. Besinis, S. D. Hadi, H. R. Le, C. Tredwin, R. D. Handy, “Antibacterial activity and biofilm inhibition by surface modified titanium alloy medical implants following application of silver, titanium dioxide and hydroxyapatite nanocoatings”, Nanotoxicology, DOI: 10.1080/17435390.2017.1299890 (2017).
W. K. Yeung, I. V. Sukhorukova, D. V. Shtansky, E. A. Levashov, I. Y. Zhitnyak, N. A. Gloushankova, P. V. Kiryukhantsev-Korneev, M. I. Petrzhik, A. Matthews, A. Yerokhin, “Characteristics and in vitro response of thin hydroxyapatite–titania films produced by plasma electrolytic oxidation of Ti alloys in electrolytes with particle additions”, The Royal Society of Chemistry Advances, 6, p. 12688 (2016).
D. Dzhurinskiy, Y.Gao, W.-K. Yeung, E. Strumban, V. Leshchinsky, P.-J.Chu, A. Matthews, A. Yerokhin, R.Gr. Maev, “Characterization and corrosion evaluation of TiO2:n-HA coatings on titanium alloy formed by plasma electrolytic oxidation”, Surface & Coatings Technology, 269, p.258 (2015).
N. Ribeiro, S.R. Sousa, C.A. van Blitterswijk, L. Moroni, F.J. Monteiro, “A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration, Biofabrication, 6(3), p. XXX (2014).
A. Zomorodian, M.P. Garcia, T. Moura e Silva, J.C.S. Fernandes, M.H. Fernandes, M.F. Montemor, “Biofunctional composite coating architectures based on polycaprolactone and nanohydroxyapatite for controlled corrosion activity and enhanced biocompatibility of magnesium AZ31 alloy”, Materials Science and Engineering C, 48, p. 434 (2014).
G. Ruphuy, J.C. Lopes, M. Dias, M.F. Barreiro, “Preparation of hydroxyapatite nanodispersions in the presence of chitosan by ultrasonication”, Conference Paper for International Conference on Biobased Materials and Composites (ICBMC), 13-16 May, Montreal, Canada (2014).
M. V. Torres, “An experimental procedure for Reaction Injection Moulding – RIM – materials formulation design”, PhD Thesis in Chemical and Biological Engineering, Department of Chemical Engineering, University of Porto (2014).
S.D. Hadi, “The Antibacterial Properties and Biocompatibility of Silver and Hydroxyapatite Nanoparticles Coating on Dental Implants”, MSc Thesis, School of Biological Sciences, Faculty of Science and Environment, University of Plymouth, UK (2014).
V. Reis, “Resposta biológica à implantação subcutânea de nanopartículas de hidroxiapatite em ratos diabéticos”, MSc Thesis Biologia Clínica Laboratorial, Universidade de Trás-os-Montes e Alto Douro (2013).
E. Pires, "Effect of the nanohydroxyapatite Formulation NanoXIM.HAp102 on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells", Integrated MSc Thesis in Bioengineering, Faculty of Engineering, University of Porto (2013).
P.A.A.P. Marques, G. Gonçalves, M.K. Singh, J. Grácio, “Graphene oxide and hydroxyapatite as fillers of polylactic acid nanocomposites: preparation and characterization.”, Journal of Nanoscience and Nanotechnology, 12, p. 6686 (2012).